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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Pecuária Sudeste. |
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Data corrente: |
04/09/2017 |
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Data da última atualização: |
16/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRITO, L. G.; NERY, L. de O.; BARBIERI, F. da S.; HUACCA, M. E. F.; PEREIRA, S. dos S.; SILVA, R. R. da; FERNANDES, C. F. C.; OLIVEIRA, M. C. de S. |
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Afiliação: |
LUCIANA GATTO BRITO, CPATU; Louí de Oliveira Néry, Bolsista CPATU; FABIO DA SILVA BARBIERI, CPATU; Maribel Elizabeth Funes Huacca, UNIR; Soraya dos Santos Pereira, Fiocruz Rondônia; RENATA REIS DA SILVA, CPATU; Carla Freire Celedonio Fernandes, Fiocruz Rondônia; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Molecular quantitative assay for esterase-mediated organophosphate resistance in Rhipicephalus microplus. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 8, n. 5, p. 725-732, 2017. |
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DOI: |
https://doi.org/10.1016/j.ttbdis.2017.05.006 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future it will be important to expand the molecular targets involved in OP resistance, which could be used for better selection of effective strategies to control cattle tick populations. MenosThe use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future ... Mostrar Tudo |
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Palavras-Chave: |
Cattle tick; Organophosphate resistance. |
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Thesaurus Nal: |
esterases. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02493naa a2200253 a 4500
001 2076221
005 2019-01-16
008 2017 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.ttbdis.2017.05.006$2DOI
100 1 $aBRITO, L. G.
245 $aMolecular quantitative assay for esterase-mediated organophosphate resistance in Rhipicephalus microplus.$h[electronic resource]
260 $c2017
520 $aThe use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future it will be important to expand the molecular targets involved in OP resistance, which could be used for better selection of effective strategies to control cattle tick populations.
650 $aesterases
653 $aCattle tick
653 $aOrganophosphate resistance
700 1 $aNERY, L. de O.
700 1 $aBARBIERI, F. da S.
700 1 $aHUACCA, M. E. F.
700 1 $aPEREIRA, S. dos S.
700 1 $aSILVA, R. R. da
700 1 $aFERNANDES, C. F. C.
700 1 $aOLIVEIRA, M. C. de S.
773 $tTicks and Tick-borne Diseases$gv. 8, n. 5, p. 725-732, 2017.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
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Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/08/2008 |
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Data da última atualização: |
03/02/2009 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
MATTA, P. M. da; SILVA, L. L. G. G. da; DIAS, P. F.; SOUTO, S. M.; AZEVEDO, B. C. de. |
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Afiliação: |
Patrícia Morais da Matta, UFRRJ; Luzimar Lamarte Gonzaga Galindo da Silva, UFRRJ; Paulo Francisco Dias, PESAGRO-EES-RIO; Sebastião Manhães Souto, Embrapa Agrobiologia; Bruno Campbell de Azevedo, UFRRJ. |
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Título: |
Influência de espécies de leguminosas arbóreas na qualidade e produção de pastagerm de capim Marandu (Brachiaria brizantha). |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 17., 2008, Rio de Janeiro. Manejo e conservação do solo e da água no contexto das mudanças ambientais. Rio de Janeiro: SBCS: Embrapa Solos: Embrapa Agrobiologia, 2008. |
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Páginas: |
4 p. |
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Descrição Física: |
1 CD-ROM. |
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Série: |
(Embrapa Solos. Documentos, 101). |
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Idioma: |
Português |
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Notas: |
Parceria: UFRRJ; PESAGRO-EES-RIO. |
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Palavras-Chave: |
Capim Marandu; Leguminosa arbórea. |
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Thesagro: |
Brachiaria Brizantha; Pastagem. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00968naa a2200241 a 4500
001 1630121
005 2009-02-03
008 2008 bl --- 0-- u #d
100 1 $aMATTA, P. M. da
245 $aInfluência de espécies de leguminosas arbóreas na qualidade e produção de pastagerm de capim Marandu (Brachiaria brizantha).
260 $c2008
300 $a4 p.$c1 CD-ROM.
490 $a(Embrapa Solos. Documentos, 101).
500 $aParceria: UFRRJ; PESAGRO-EES-RIO.
650 $aBrachiaria Brizantha
650 $aPastagem
653 $aCapim Marandu
653 $aLeguminosa arbórea
700 1 $aSILVA, L. L. G. G. da
700 1 $aDIAS, P. F.
700 1 $aSOUTO, S. M.
700 1 $aAZEVEDO, B. C. de
773 $tIn: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 17., 2008, Rio de Janeiro. Manejo e conservação do solo e da água no contexto das mudanças ambientais. Rio de Janeiro: SBCS: Embrapa Solos: Embrapa Agrobiologia, 2008.
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Embrapa Agrobiologia (CNPAB) |
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